Two-time Wimbledon champion had defeated Greet Minnen in her opening match
Two-time Wimbledon champion Kvitova, who reached the semi-finals at Roland Garros in 2020 and 2012, beat Belgian qualifier Greet Minnen 6-7(3) 7-6(5) 6-1 in her opening match of the tournament and hurt her ankle shortly afterwards.
“It is with great disappointment that I announce my withdrawal from Roland Garros,” the 31-year-old Kvitova said on Twitter on Tuesday. “During my post-match press requirements on Sunday I fell and hurt my ankle.
“Unfortunately after an MRI and much discussion with my team, I have made the tough decision that it would be unwise to play on it. It’s incredibly bad luck, but I will stay strong and do my best to recover in time for the grasscourt season.”
Kvitova was due to take on unseeded Russian Elena Vesnina in the second round.