Police said around 4,000 pairs were taken in an overnight raid at Abington Services Interchange on the M74 in South Lanarkshire.
Around £400,000 worth of Nike branded footwear has been stolen from a lorry at motorway services.
The theft happened between 5pm on Wednesday and 4.30am on Thursday.
The vehicle and two others parked nearby were damaged during the theft and police are appealing for information.
Detective Constable Heather Anderson of Wishaw CID said: “Inquéritos into this incident are ongoing and officers are checking for CCTV footage to try to establish descriptions of those responsible.
“I would urge anyone who has information or who saw anything suspicious to contact Police Scotland.
“No matter how insignificant it may have seemed at the time, it could be vital to our investigation.
“In particular, I would ask motorists with dashcams who were in the area at the time to check their footage. It is possible that it could have recorded something which could assist in our ongoing inquiries.”
Anyone with information is asked to call Police Scotland on 101, quoting incident number 0308 of February 24, or Crimestoppers on 0800 555 111 where anonymity can be maintained.