The 34-year-old had a scan on Tuesday.
Enquanto isso, Gerente Mauricio Pochettino who insisted during his pre-match press conference that there is no rift between he and the former Barcelona man after he appeared puzzled by his withdrawal, has left him out of the 22-man travelling party.
Messi’s exclusion will not have gone unnoticed at Manchester City, who are due at the Parc des Princes in the Champions League next Tuesday night.
The much-decorated Argentina international joined PSG during the summer after his 21-year stay at the Nou Camp came to an end.
He made his debut as a substitute in a 2-0 win at Reims on August 29, but is yet to score for his new club in three appearances.