The Scot criticised the world number three over lengthy bathroom breaks between sets.
The 34-year-old Scot was livid at the lengthy bathroom breaks Tsitsipas was taking between sets and demanded more should be done to clamp down on the “nonsense”.
“It’s not so much leaving the court. It’s the amount of time,” said Murray.
“I spoke to my team before the match about it and said to expect that, prepare for it if things were not going his way. So I was trying to do that.
“But the issue is that you cannot stop the way that that affects you physically. When you’re playing a brutal match like that, you know, stopping for seven, eight minutes, you do cool down.
“It’s just disappointing because I feel it influenced the outcome of the match. I’m not saying I necessarily win that match, 当然, but it had influence on what was happening after those breaks.
“I rate him a lot. I think he’s a brilliant player. I think he’s great for the game. But I have zero time for that stuff at all, and I lost respect for him.
“That’s what I feel. But if everyone else feels like that’s totally cool and there’s no issue with it, then maybe I’m the one being unreasonable.
“But I think it’s nonsense. And he knows it, as well.”
Murray came so close to the biggest win of his comeback, almost defying the odds, the heat and a slippery pair of shoes as well as the borderline gamesmanship from the Greek third seed.
But in a match lasting just short of five hours, the two-time Wimbledon champion went down 2-6 6-7 (7) 3-6 6-3 6-4.
Having won the first set – the first he had taken from a top-five player since 2017 – Murray had two points to clinch the second in the tie-break.
But drenched in sweat in the humid conditions, he began complaining that his trainers had become too slippery – yet it soon became apparent he did not have a spare pair.
“是的, that’s my bad, but I’ve never had that happen in a match,“ 他加了.
然而, having come through career-saving hip surgery and a host of other injury problems over the last few years, Murray was not about to let a pair of sweaty pumps derail him.
He took the next set to lead 2-1 but Tsitsipas upped his level, racing 5-0 ahead then taking the match to a deciding set.
When Tsitsipas disappeared for yet another off-court break before the start of the fifth he was given a time violation, but Murray was clearly wound up.
After dropping serve in the opening game he was heard muttering about “cheating” and later asked supervisor Gerry Armstrong why it was taking Tsitsipas so long.
“What’s he doing in there? It’s never taken me that long to go to the toilet,” he moaned.
Feeding off the crowd and a familiar sense of injustice, Murray forced a break point at 1-2 but it was extinguished by a sizzling Tsitsipas forehand.
Tsitsipas held his nerve along the finishing straight and when an exhausted Murray dropped a forehand into the net, his latest Flushing Meadows campaign was over.
He concluded: “Overall I did well tonight, but I’m really, really disappointed, really disappointed after that, frustrated, all those things. Really disappointed.”